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Hippocampal Neuronal Cultures to Detect and Study New Pathogenic Antibodies Involved in Autoimmune Encephalitis
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Hippocampal Neuronal Cultures to Detect and Study New Pathogenic Antibodies Involved in Autoimmune Encephalitis

Hippocampal Neuronal Cultures to Detect and Study New Pathogenic Antibodies Involved in Autoimmune Encephalitis

English

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2,679 Views

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08:20 min

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June 02, 2022

DOI:

10.3791/63829-v

DOI:
10.3791/63829-v

•

08:20 min
June 02, 2022

•

13 Views
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1ICFO-Institut de Ciències Fotòniques,The Barcelona Institute of Science and Technology, 2Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Hospital Clínic,Universitat de Barcelona

Summary

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Autoimmune encephalitis is a new category of antibody-mediated diseases of the central nervous system. Hippocampal neurons can be used to discover and characterize these antibodies. This article provides a protocol for primary cell culture and immunostaining to determine autoantibodies in the serum and cerebrospinal fluid of patients.

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Cite this Article

Cunquero, M., Aguilar, E., Loza-Alvarez, P., Planagumà, J. Hippocampal Neuronal Cultures to Detect and Study New Pathogenic Antibodies Involved in Autoimmune Encephalitis. J. Vis. Exp. (184), e63829, doi:10.3791/63829 (2022).

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