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从人包皮中分离、培养和表征原代施旺细胞、角质形成细胞和成纤维细胞
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Neuroscience
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JoVE Journal Neuroscience
Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin
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从人包皮中分离、培养和表征原代施旺细胞、角质形成细胞和成纤维细胞

DOI:
10.3791/63776-v

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10:36 min

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March 23, 2022

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, , ,
1Department of Orthopaedics and Rehabilitation, Center for Orthopaedic Research and Translational Science (CORTS),The Pennsylvania State University College of Medicine, 2Department of Dermatology,The Pennsylvania State University College of Medicine

Chapters

  • 00:04Introduction
  • 01:17Epidermis and Dermis Separation Procedure
  • 03:03Keratinocyte Isolation from the Epidermis
  • 05:02Isolation of Schwann Cells (SCs) and Fibroblast from the Dermis
  • 08:18Results: Characterization of Human Foreskin-Derived Primary Keratinocytes, Schwann Cells, and Fibroblasts
  • 09:43Conclusion

Summary

Automatic Translation

Automatic Translation

研究与肌肉骨骼损伤相关的伤口愈合通常需要评估施万细胞（SCs）、角质形成细胞和成纤维细胞之间的 体外 相互作用。该协议描述了从人包皮中分离，培养和表征这些原代细胞。

Tags

Primary Schwann CellsKeratinocytesFibroblastsHuman ForeskinSkin CellsCell IsolationCell CultureCell InteractionsSkin HomeostasisSkin PathophysiologyWound HealingSkin Disease

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