The present protocol demonstrates the different steps involved in wounding the cornea of an embryonic chick in ovo. The regenerating or fully restored corneas can be analyzed for regenerative potential using various cellular and molecular techniques following the wounding procedure.
Pathuri, M., Spurlin III, J., Lwigale, P., Schwend, T. Investigating Scarless Tissue Regeneration in Embryonic Wounded Chick Corneas. J. Vis. Exp. (183), e63570, doi:10.3791/63570 (2022).