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Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

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13:21 min

April 06, 2022

DOI:

13:21 min
April 06, 2022

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Summary

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The protocol describes high-throughput spheroid generation for bioprinting using the multi-parametric analysis of their oxygenation and cell death on a standard fluorescence microscope. This approach can be applied to control the spheroids viability and perform standardization, which is important in modeling 3D tissue, tumor microenvironment, and successful (micro)tissue biofabrication.

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