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Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

English

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3,063 Views

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13:21 min

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April 06, 2022

DOI:

10.3791/63403-v

DOI:
10.3791/63403-v

•

13:21 min
April 06, 2022

•

7 Views
, , , ,
1Tissue Engineering and Biomaterials Group, Department of Human Structure and Repair, Faculty of Medical and Health Sciences,Ghent University, 2Health and Happiness (H&H) Group,National Food Innovation Hub, 3Institute of Analytical Chemistry and Food Chemistry,Graz University of Technology

Summary

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The protocol describes high-throughput spheroid generation for bioprinting using the multi-parametric analysis of their oxygenation and cell death on a standard fluorescence microscope. This approach can be applied to control the spheroids viability and perform standardization, which is important in modeling 3D tissue, tumor microenvironment, and successful (micro)tissue biofabrication.

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Cite this Article

Okkelman, I. A., Vercruysse, C., Kondrashina, A. V., Borisov, S. M., Dmitriev, R. I. Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids. J. Vis. Exp. (182), e63403, doi:10.3791/63403 (2022).

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