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February 11, 2022
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1Center for Innovative Medical Devices and Sensors (REDDI Lab),Clemson University, 2Department of Bioengineering,Clemson University, 3Swann Medicine, 4Student Health Services,Clemson University, 5Department of Chemical & Biomolecular Engineering,Clemson University, 6Department of Chemical & Biomolecular Engineering,University of Delaware
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このプロトコルは、オープンソースの自動化を利用して唾液サンプルのRT-qPCR分子検査を実行するSARS-CoV-2診断方法を記述しています。このスケーラブルなアプローチは、臨床公衆衛生サーベイランスや小規模な大学研究所の能力向上にも適用できます。
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Keywords Extracted From The Given Text:SARS-CoV-2Quantitative Reverse Transcriptase PCRSaliva DiagnosticOpen-source Pipetting RobotsCOVID-19 DiagnosticsSaliva-based TestingAutomated Parallel WorkflowsSample DecontaminationHeat TreatmentSample LoadingSample Assignment3D Printed RacksMaster Mix PlatesPython Operating Protocol
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Cite this Article
Ham, R. E., Smothers, A. R., King, K. L., Napolitano, J. M., Swann, T. J., Pekarek, L. G., Blenner, M. A., Dean, D. Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots. J. Vis. Exp. (180), e63395, doi:10.3791/63395 (2022).
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