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Measurement of Oxygen Consumption Rate in Acute Striatal Slices from Adult Mice
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Neuroscience
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JoVE Journal Neuroscience
Measurement of Oxygen Consumption Rate in Acute Striatal Slices from Adult Mice

Measurement of Oxygen Consumption Rate in Acute Striatal Slices from Adult Mice

English

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2,828 Views

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07:41 min

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June 08, 2022

DOI:

10.3791/63379-v

DOI:
10.3791/63379-v

•

07:41 min
June 08, 2022

•

38 Views
, , , , , , , ,
1Department of Neuroscience,Thomas Jefferson University, 2School of Biomedical Engineering,Drexel University, 3Department of Pathology, MitoCare Center,Thomas Jefferson University, 4School of Life Sciences,Peking University

Summary

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Oxygen consumption rate (OCR) is a common proxy for mitochondrial function and can be used to study different disease models. We developed a new method using a Seahorse XF analyzer to directly measure the OCR in acute striatal slices from adult mice that is more physiologically relevant than other methods.

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Cite this Article

Zhi, L., Zhao, J., Jaffe, D., Chen, Y., Wang, N., Qin, Q., Seifert, E. L., Li, C., Zhang, H. Measurement of Oxygen Consumption Rate in Acute Striatal Slices from Adult Mice. J. Vis. Exp. (184), e63379, doi:10.3791/63379 (2022).

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