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Measurement of Oxygen Consumption Rate in Acute Striatal Slices from Adult Mice
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JoVE Journal Neurowissenschaften
Measurement of Oxygen Consumption Rate in Acute Striatal Slices from Adult Mice

Measurement of Oxygen Consumption Rate in Acute Striatal Slices from Adult Mice

DOI:
10.3791/63379-v

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07:41 min

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June 08, 2022

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, , , , , , , ,
1Department of Neuroscience,Thomas Jefferson University, 2School of Biomedical Engineering,Drexel University, 3Department of Pathology, MitoCare Center,Thomas Jefferson University, 4School of Life Sciences,Peking University

Kapitel

  • 00:04Introduction
  • 00:57Hydrating the Cartridge Sensors
  • 01:45Preparing the Tissue Plate
  • 02:24Acute Striatal Slice Preparation and Excised Slice Placement
  • 04:11Loading of Cartridges with Desired Compounds
  • 05:17Calibration and Performing the Assay
  • 06:07Results: Measuring the Oxygen Consumption Rates in Acute Striatal Slices from Adult Mice
  • 07:11Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Oxygen consumption rate (OCR) is a common proxy for mitochondrial function and can be used to study different disease models. We developed a new method using a Seahorse XF analyzer to directly measure the OCR in acute striatal slices from adult mice that is more physiologically relevant than other methods.

Tags

Oxygen Consumption RateAcute Striatal SlicesAdult MiceSeahorse XF AnalyzerMitochondrial FunctionParkinson’s DiseaseHuntington’s DiseaseExtracellular Flux AssayArtificial Cerebral Spinal FluidVibratomeCoronal Striatal SlicesRespiration Buffer

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