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Isolamento delle cellule staminali mesenchimali del tessuto adiposo di ratto per la differenziazione in cellule produttrici di insulina
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells
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Isolamento delle cellule staminali mesenchimali del tessuto adiposo di ratto per la differenziazione in cellule produttrici di insulina

italiano

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6,377 Views

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08:09 min

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August 29, 2022

DOI:

10.3791/63348-v

DOI:
10.3791/63348-v

•

08:09 min
August 29, 2022

•

14 Views
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1Biochemistry Department, Faculty of Pharmacy,Ain Shams University, 2Pharmacology and Biochemistry Department, Faculty of Pharmacy,The British University in Egypt, 3Center for Drug Research and Development (CDRD), Faculty of Pharmacy,The British University in Egypt

Summary

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Le cellule staminali mesenchimali derivate dal tessuto adiposo (Ad-MSC) possono essere una potenziale fonte di MSC che si differenziano in cellule produttrici di insulina (IPC). In questo protocollo, forniamo passaggi dettagliati per l'isolamento e la caratterizzazione di Ad-MSC epididimali di ratto, seguiti da un protocollo semplice e breve per la generazione di IPC dallo stesso Rat Ad-MSCs.

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Cite this Article

Kassem, D. H., Habib, S. A., Badr, O. I., Kamal, M. M. Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells. J. Vis. Exp. (186), e63348, doi:10.3791/63348 (2022).

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