Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

Mark C, Wagner; Ruben M. Sandoval; Silvia B. Campos-Bilderback; Bruce A. Molitoris

doi:10.3791/63329

Journal

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Medicine

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Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

JoVE Journal
Medicine

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JoVE Journal Medicine

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

1,929 Views

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11:47 min

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March 04, 2022

DOI:

10.3791/63329-v

DOI:

10.3791/63329-v

•

11:47 min

March 04, 2022

•

2 Views

Mark C, Wagner¹, Ruben M. Sandoval¹, Silvia B. Campos-Bilderback¹, Bruce A. Molitoris¹

¹Department of Medicine, Division of Nephrology,Indiana University School of Medicine

Summary

Here, we present a protocol using 2-photon microscopy in Munich Wistar Fromter rats with surface glomeruli to quantifythe effects of prolonged ureteral obstruction on glomerular dynamics and function.

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2-photon Microscopy Unilateral Ureteral Obstruction Kidney Function Inflammation Fibrosis Cell-cell Interactions Subcellular Events Ureteral Procedure Anesthetize Renal Pedicle Ureter Suture Abdominal Incision Kidney Position Muscle Layer Hemostats

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Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

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