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卡利内克特氏菌中神经肽的多方面质谱研究
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Chemistry
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JoVE Journal Chemistry
Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus
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卡利内克特氏菌中神经肽的多方面质谱研究

English

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2,272 Views

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09:22 min

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May 31, 2022

DOI:

10.3791/63322-v

DOI:
10.3791/63322-v

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09:22 min
May 31, 2022

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2 Views
, ,
1Department of Chemistry,University of Wisconsin-Madison, 2School of Pharmacy,University of Wisconsin-Madison

Summary

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神经肽的质谱表征提供序列、定量和定位信息。这种优化的工作流程不仅对神经肽研究有用，而且对其他内源性肽也很有用。此处提供的方案描述了使用 LC-ESI-MS、MALDI-MS 点斑和 MALDI-MS 成像的神经肽的样品制备、MS 采集、MS 分析和神经肽的数据库生成。

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NeuropeptidesMass SpectrometryCallinectes SapidusCrustaceanSample PreparationDesaltingQuantitationLocalizationBiomarkers

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Phetsanthad, A., Vu, N. Q., Li, L. Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus. J. Vis. Exp. (183), e63322, doi:10.3791/63322 (2022).

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