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Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus
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JoVE 杂志 化学
Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus

Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus

DOI:
10.3791/63322-v

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09:22 min

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May 31, 2022

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, ,
1Department of Chemistry,University of Wisconsin-Madison, 2School of Pharmacy,University of Wisconsin-Madison

Chapters

  • 00:04Introduction
  • 00:49LC-ESI-MS Analysis of Neuropeptides
  • 02:45MALDI-MS Spotting Analysis of Neuropeptides
  • 04:13MALDI-MS Imaging Analysis of Neuropeptides
  • 05:36Discovering Novel Putative Neuropeptides Using De Novo Sequencing and Transcriptome Mining for Predicted Neuropeptide Sequences
  • 06:57Results: De Novo Sequencing and MALDI-MS Imaging of Neuropeptides
  • 08:41Conclusion

Summary

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Mass spectrometric characterization of neuropeptides provides sequence, quantitation, and localization information. This optimized workflow is not only useful for neuropeptide studies, but also other endogenous peptides. The protocols provided here describe sample preparation, MS acquisition, MS analysis, and database generation of neuropeptides using LC-ESI-MS, MALDI-MS spotting, and MALDI-MS imaging.

Tags

NeuropeptidesMass SpectrometryCallinectes SapidusCrustaceanSample PreparationDesaltingQuantitationLocalizationBiomarkers

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