Journal
/
Engineering
/
Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads
JoVE Journal
Engineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Engineering
Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads

Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads

English

Automatically Generated

2,222 Views

•

06:08 min

•

October 17, 2022

DOI:

10.3791/63300-v

DOI:
10.3791/63300-v

•

06:08 min
October 17, 2022

•

6 Views
, , ,
1Department of Digital Anti-Aging Health Care,Inje University, 2Micro device Lab Co., Ltd, 3Biohealth Products Research Center (BPRC),Inje University, 4Department of Biomedical Engineering,Inje University

Summary

Automatically generated

This paper describesthe fabrication and operation of microfluidic acoustophoretic chips using the microfluidic acoustophoresis technique and aptamer-modified microbeads that can be used for fast, efficient isolation of Gram-negative bacteria from a medium.

Explore More Videos

Microfluidic AcoustophoresisGram-negative Bacteria SeparationAptamer Affinity BeadsFlowthrough SeparationCell IsolationMicrofluidic DevicePZTBiotinylated DNA AptamerStreptavidin-coated MicrobeadsLuria-Bertani MediumCentrifugationTris-HCl BufferBSA

Read Article

Cite this Article

Choi, H. J., Kim, B. W., Lee, S., Jeong, O. C. Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads. J. Vis. Exp. (188), e63300, doi:10.3791/63300 (2022).

Download .ris file

Copy

Share Video

.

Copy