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12:19 min
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April 22, 2022
DOI:
1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine,National Taiwan University, 2Department of Laboratory Medicine,National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics,The Ohio State University
Summary
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우라실-DNA 글리코실라제 활성을 분석하기 위한 비표지, 비-방사성-동위원소 방법은 직접 아푸린산/아피리미딘 부위 함유 생성물 분석을 위해 MALDI-TOF 질량 분광법을 사용하여 개발되었다. 이 분석은 DNA 글리코실라제 측정에 매우 간단하고, 구체적이며, 빠르고, 사용하기 쉬운 것으로 판명되었습니다.
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Chang, H., Su, K., Goodman, S. D., Cheng, W., Lin, L., Yang, Y., Chang, S., Fang, W. Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis. J. Vis. Exp. (182), e63089, doi:10.3791/63089 (2022).
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