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Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease
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Medicine
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JoVE Journal Medicine
Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease

Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease

English

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2,152 Views

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12:15 min

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February 08, 2022

DOI:

10.3791/62909-v

DOI:
10.3791/62909-v

•

12:15 min
February 08, 2022

•

4 Views
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1Centre de recherche Cardio-Thoracique de Bordeaux,Univ. Bordeaux, 2IHU Liryc, Electrophysiology and Heart Modeling Institute,Fondation Bordeaux Univ., 3Electrophysiology and Ablation Unit,Bordeaux University Hospital (CHU)

Summary

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Here, we present a protocol to obtain high resolution micro computed tomography images of healthy and pathological large mammalian whole hearts with collagen-selective contrast enhancement.

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Pallares-Lupon, N., Bayer, J. D., Guillot, B., Caluori, G., Ramlugun, G. S., Kulkarni, K., Loyer, V., Bloquet, S., El Hamrani, D., Naulin, J., Constantin, M., Dos Santos, P., Bernus, O., Jaïs, P., Pasdois, P., Walton, R. D. Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease. J. Vis. Exp. (180), e62909, doi:10.3791/62909 (2022).

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