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Preparação de grades de amostras de alta temperatura para crio-EM
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Biochemistry
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JoVE Journal Biochemistry
Preparation of High-Temperature Sample Grids for Cryo-EM

Preparação de grades de amostras de alta temperatura para crio-EM

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3,240 Views

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05:05 min

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July 26, 2021

DOI:

10.3791/62772-v

DOI:
10.3791/62772-v

•

05:05 min
July 26, 2021

•

9 Views
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1Institute of Biological Chemistry,Academia Sinica, 2Academia Sinica Cryo-EM Facility,Academia Sinica, 3Department of Life Sciences,National Central University, 4Hubei University, 5Institute of Biochemical Sciences,National Taiwan University

Summary

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Este artigo fornece um protocolo detalhado para a preparação de grades de amostras a temperaturas tão altas quanto 70 °C, antes do congelamento por imersão para experimentos de crio-EM.

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Cite this Article

Chang, Y., Chen, C., Tsai, M. Preparation of High-Temperature Sample Grids for Cryo-EM. J. Vis. Exp. (173), e62772, doi:10.3791/62772 (2021).

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