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May 13, 2021
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In Drosophila, the oocyte nucleus undergoes microtubule-dependent migration during oogenesis. Here, we describe a protocol that was developed to follow the migration by performing live imaging on egg chambers ex-vivo. Our procedure maintains egg chambers alive for 12 h to acquire multi-position 3D time-lapse movies using spinning-disk confocal microscopy.
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Loh, M., Guichet, A., Bernard, F. Nuclear Migration in the Drosophila Oocyte. J. Vis. Exp. (171), e62688, doi:10.3791/62688 (2021).
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