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使用 D11 scFv-碱性磷酸酶融合蛋白和免疫荧光直接检测组织中的异左旋蛋白
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence
Please note that all translations are automatically generated.
Click here for the English version.
使用 D11 scFv-碱性磷酸酶融合蛋白和免疫荧光直接检测组织中的异左旋蛋白
DOI:
10.3791/62603-v
•
06:33 min
•
July 05, 2021
•
Cassandra Warden*
1
,
Alan J. Simmons*
2
,
Lejla Pasic
,
Ashley Pitzer
6
,
Sean S. Davies
,
Justin H. Layer
,
Raymond L. Mernaugh
,
Annet Kirabo
6
1
Vanderbilt Eye Institute
,
Vanderbilt University Medical Center
,
2
Department of Cell and Developmental Biology
,
Vanderbilt University
,
3
Department of Biochemistry
,
Vanderbilt University
,
4
Division of Clinical Pharmacology, Department of Medicine
,
Vanderbilt University Medical Center
,
5
Division of Hematology and Oncology
,
Indiana University School of Medicine
,
6
Department of Molecular Physiology and Biophysics
,
Vanderbilt University
Chapters
00:05
Introduction
00:54
Immunofluorescence
03:21
Negative Controls and Competitive Assays
04:37
Results: Detection of IsoLGs in Hypertensive and Normotensive Mice and Human Tissues
06:08
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文提供了使用碱性磷酸酶偶联的 ScFv D11 抗体通过免疫荧光测量组织中异左旋前列腺素的详细方法。小鼠和人类的高血压模型用于解释与组织样本中异左旋蛋白测量相关的分步程序和基本原理。
Tags
Isolevuglandins
D11 ScFv-alkaline Phosphatase
Immunofluorescence
Cardiovascular Disease
Antigen Retrieval
Alkaline Phosphatase Development
Immunohistochemistry
Fluorescent Microscopy
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