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मानव माइलॉयड यू 937 कोशिकाओं में फ्लो साइटोमेट्री द्वारा SAMHD1 प्रतिबंध का विश्लेषण
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells
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मानव माइलॉयड यू 937 कोशिकाओं में फ्लो साइटोमेट्री द्वारा SAMHD1 प्रतिबंध का विश्लेषण

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09:43 min

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June 13, 2021

DOI:

10.3791/62502-v

DOI:
10.3791/62502-v

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09:43 min
June 13, 2021

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14 Views
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1Retrovirus-Host Interactions Laboratory,The Francis Crick Institute, 2Retroviral Replication Laboratory,The Francis Crick Institute, 3Sidney Sussex College, Department of Medicine,University of Cambridge

Tags

SAMHD1Flow CytometryU937 CellsHIV InfectionMacrophagesCellular FactorsMulticolor Flow CytometryCytometerFlow RateForward ScatterSide ScatterYFPRFPCompensation Controls

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Cite this Article

Ordonez, P., Bishop, K. N., Stoye, J. P., Groom, H. C. T. Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells. J. Vis. Exp. (172), e62502, doi:10.3791/62502 (2021).

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