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Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells

Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells

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09:43 min

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June 13, 2021

DOI:

10.3791/62502-v

DOI:
10.3791/62502-v

•

09:43 min
June 13, 2021

•

27 Views
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1Retrovirus-Host Interactions Laboratory,The Francis Crick Institute, 2Retroviral Replication Laboratory,The Francis Crick Institute, 3Sidney Sussex College, Department of Medicine,University of Cambridge

Summary

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Described here is an established method to determine the extent of HIV-1 restriction by the cellular inhibitory protein SAMHD1. Human myeloid lineage U937 cells are transduced with a SAMHD1 expression vector co-expressing YFP, differentiated and then challenged with HIV-RFP. The level of restriction is determined by flow cytometry analysis.

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Cite this Article

Ordonez, P., Bishop, K. N., Stoye, J. P., Groom, H. C. T. Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells. J. Vis. Exp. (172), e62502, doi:10.3791/62502 (2021).

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