JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Journal
/
Immunology and Infection
/
高通量抗病毒检测筛查寨卡病毒复制抑制剂
JoVE Journal
Immunology and Infection
Author Produced
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication
Please note that all translations are automatically generated.
Click here for the English version.
高通量抗病毒检测筛查寨卡病毒复制抑制剂
DOI:
10.3791/62422-v
•
10:16 min
•
October 30, 2021
•
Rafaela S. Fernandes
,
Gabriela D. Noske
,
Victor O. Gawriljuk
,
Ketllyn I. Z. de Oliveira
,
Andre S. Godoy
,
Nathalya C. M. R. Mesquita
,
Glaucius Oliva
1
São Carlos Institute of Physics
,
University of Sao Paulo
Chapters
00:02
Introduction
01:44
Luciferase Activity Assay
03:53
Cell Proliferation-Based MTT Assay
04:27
NS2B-NS3 Protease Activity Assay
05:19
NS5 RdRp Elongation Assay
06:32
Representative Results
08:06
Conclusions
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这项研究中,我们描述了用于基于雷普利康和病毒酶的检测的协议,以高通量筛选格式筛选寨卡病毒复制的抑制剂。
Tags
Zika Virus
Antiviral Assay
High-throughput Screening
Replicon
Viral Enzyme
Renilla Luciferase
NS3 Protease
NS5 RNA-dependent RNA Polymerase
RdRp
BHK-21 Cell Line
Cell Culture
Trypsinization
Compound Screening
DMEM
DMSO
Positive Control
Negative Control
Renilla Luciferase Lysis Buffer
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
解剖宿主 - 病毒相互作用模型疱疹病毒裂解性复制
流感病毒的高通量检测方法
高通量筛选RNA病毒的广谱化学抑制剂
一个协议,用于分析丙型肝炎病毒复制
高通量检测到的表型<em>沙门氏菌</em>伤寒协会,侵袭和复制的巨噬细胞
早期的病毒进入测定的鉴定和抗病毒化合物的评价
使用人类流感病毒感染的斑马鱼模型到屏幕抗病毒药物和表征宿主免疫细胞反应
来自新世界Zika病毒感染性克隆的重组病毒的救援和表征
Zika 病毒特定诊断表位发现
高通量的高效液氧活化病毒膜融合检测 HIV-1 病毒膜融合抑制剂的研究
Read Article