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膜結合型IL-21修飾B細胞株を用いたナチュラルキラー(NK)およびCAR-NK細胞増殖法
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Natural Killer (NK) and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line
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膜結合型IL-21修飾B細胞株を用いたナチュラルキラー(NK)およびCAR-NK細胞増殖法

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4,700 Views

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11:02 min

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February 08, 2022

DOI:

10.3791/62336-v

DOI:
10.3791/62336-v

•

11:02 min
February 08, 2022

•

17 Views
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1Department of Pathology, Immunology and Laboratory Medicine,Rutgers-New Jersey Medical School, 2Department of Microbiology, Biochemistry & Molecular Genetics, Public Health Research Institute Center, New Jersey Medical School,Rutgers University, 3Center for Immunity and Inflammation, New Jersey Medical School,Rutgers-The State University of New Jersey

Summary

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ここでは、末梢血ナチュラルキラー(PBNK)、肝臓組織からのNK細胞、末梢血単核球(PBMC)または臍帯血(CB)に由来するキメラ抗原受容体(CAR)-NK細胞を拡大する方法を紹介します。このプロトコルは、増殖したNK細胞の最適化された純度に加えて、221-mIL-21フィーダー細胞を使用したNKおよびCAR-NK細胞の増殖を実証します。

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Ma, M., Badeti, S., Kim, J. K., Liu, D. Natural Killer (NK) and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line. J. Vis. Exp. (180), e62336, doi:10.3791/62336 (2022).

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