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Microscopia confocal de varredura a laser da dinâmica do cálcio em fatias agudas de tecido pancreático do rato
JoVE Journal
Biology
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JoVE Journal Biology
Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices

Microscopia confocal de varredura a laser da dinâmica do cálcio em fatias agudas de tecido pancreático do rato

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3,421 Views

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10:49 min

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April 13, 2021

DOI:

10.3791/62293-v

DOI:
10.3791/62293-v

•

10:49 min
April 13, 2021

•

11 Views
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1Institute of Physiology, Faculty of Medicine,University of Maribor, 2Faculty of Natural Sciences and Mathematics,University of Maribor, 3Center for Physiology and Pharmacology,Medical University of Vienna

Summary

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Apresentamos a preparação de fatias agudas de tecido pancreático e seu uso em microscopia de escaneamento a laser confocal para estudar a dinâmica do cálcio simultaneamente em um grande número de células vivas, durante longos períodos de tempo, e com alta resolução espástica.

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Cite this Article

Stožer, A., Dolenšek, J., Križančić Bombek, L., Pohorec, V., Slak Rupnik, M., Klemen, M. S. Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices. J. Vis. Exp. (170), e62293, doi:10.3791/62293 (2021).

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