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Test di imaging quantitativo in vitro per la fagocitosi delle cellule morte del neuroblastoma da parte di iPSC-Macrophages
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages
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Test di imaging quantitativo in vitro per la fagocitosi delle cellule morte del neuroblastoma da parte di iPSC-Macrophages

italiano

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5,013 Views

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09:48 min

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February 14, 2021

DOI:

10.3791/62217-v

DOI:
10.3791/62217-v

•

09:48 min
February 14, 2021

•

10 Views
, , , ,
1James Martin Stem Cell Facility, Sir William Dunn School of Pathology,University of Oxford, 2Alzheimer’s Research UK Oxford Drug Discovery Institute, Nuffield Department of Medicine Research Building,University of Oxford, 3UK Dementia Research Institute,Cardiff University

Summary

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Le malattie neurodegenerative sono associate a funzioni microglia disregolate. Questo articolo delinea un test in vitro della fagocitosi delle cellule di neuroblastoma da parte dei macrofagi iPSC. Le lettura quantitative al microscopio sono descritte sia per l'imaging time-lapse a cellule vive che per l'imaging ad alto contenuto a cellule fisse.

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Cite this Article

Hall-Roberts, H., Di Daniel, E., James, W. S., Davis, J. B., Cowley, S. A. In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages. J. Vis. Exp. (168), e62217, doi:10.3791/62217 (2021).

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