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In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal
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Neuroscience
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JoVE Journal Neuroscience
In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal

In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal

4,239 Views

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05:25 min

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February 10, 2021

DOI:

10.3791/62166-v

DOI:
10.3791/62166-v

•

05:25 min
February 10, 2021

•

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1Cellular and Molecular Neurobiology, Zoological Institute,Technische Universität Braunschweig, 2Institute of Pathophysiology,University Medical Center of the Johannes Gutenberg University, 3Cell Physiology, Zoological institute,Technische Universität Braunschweig

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In Vivo ImagingZebrafish LarvaeZebrafish JuvenilesSkull RemovalSkin RemovalNeuronal ArchitecturePigment CellsLarval DevelopmentSynaptic PlasticityNeuron DegenerationNeuron RegenerationACSFAgarose EmbeddingAnesthesiaGlass NeedleSkin IncisionBrain Exposure

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Schramm, P., Hetsch, F., Meier, J. C., Köster, R. W. In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal. J. Vis. Exp. (168), e62166, doi:10.3791/62166 (2021).

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