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SITUハイブリダイゼーションにおける免疫RNA-蛍光を用いたSARS-CoV-2の可視化
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Immunology and Infection
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JoVE Journal Immunology and Infection
Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
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SITUハイブリダイゼーションにおける免疫RNA-蛍光を用いたSARS-CoV-2の可視化

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5,828 Views

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05:23 min

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December 23, 2020

DOI:

10.3791/62067-v

DOI:
10.3791/62067-v

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05:23 min
December 23, 2020

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2 Views
, , , , , , ,
1Malopolska Centre of Biotechnology,Jagiellonian University, 2Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology,Jagiellonian University, 3Department of Cardiac, Vascular and Endovascular Surgery and Transplantology,The Medical University of Silesia in Katowice, 4Silesian Centre for Heart Diseases

Summary

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ここでは、その蛍光におけるRNA蛍光をその蛍光(RNA-FISH)と免疫蛍光と組み合わせて、重症急性呼吸器症候群コロナウイルス2(SARS-CoV-2)RNAを可視化する簡単な方法を説明する。このプロトコルは、単一細胞レベルでのSARS-CoV-2 RNA-宿主相互作用の分子特性の理解を深める可能性がある。

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Cite this Article

Kula-Pacurar, A., Wadas, J., Suder, A., Szczepanski, A., Milewska, A., Ochman, M., Stacel, T., Pyrc, K. Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization. J. Vis. Exp. (166), e62067, doi:10.3791/62067 (2020).

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