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Developmental Biology

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Accurate Follicle Enumeration in Adult Mouse Ovaries

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Developmental Biology

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JoVE Journal Developmental Biology

Accurate Follicle Enumeration in Adult Mouse Ovaries

Accurate Follicle Enumeration in Adult Mouse Ovaries

DOI:

10.3791/61782-v

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07:27 min

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October 16, 2020

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Amy L. Winship, Urooza C. Sarma, Lauren R. Alesi, Karla J. Hutt

¹Development and Stem Cells Program, Monash Biomedicine Discovery Institute, and Department of Anatomy and Developmental Biology,Monash University

Chapters

00:05Introduction
01:07Stereological Primordial Follicle Number Estimation
04:36Direct Ovarian Follicle Count Primordial Follicle Number Estimation
05:33Results: Representative Optical Fractionator and Direct Counting Method Comparison for Mouse Primordial Follicle Evaluation
06:22Conclusion

Summary

Automatic Translation

Here, we describe, compare, and contrast two different techniques for accurate follicle counting of fixed mouse ovarian tissues.

Tags

Keywords: Stereology Follicle Enumeration Ovarian Biology Ovarian Reserves Optical Fractionator Primordial Follicle Histological Techniques Morphology Reproducibility

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