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An Efficient Method for Adenovirus Production
JoVE Journal
Bioengineering
Author Produced
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JoVE Journal Bioengineering
An Efficient Method for Adenovirus Production

An Efficient Method for Adenovirus Production

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10:06 min

June 10, 2021

DOI:

10:06 min
June 10, 2021

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Summary

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Here, we present a protocol for adenovirus production using the pAdEasy system. The technology includes the recombination of the pAdTrack and pAdEasy-1 plasmids, the adenovirus packaging and amplification, the purification of the adenoviral particles from cell lysate and culture medium, the viral titration, and the functional testing of the adenovirus.

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