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June 10, 2021
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Here, we present a protocol for adenovirus production using the pAdEasy system. The technology includes the recombination of the pAdTrack and pAdEasy-1 plasmids, the adenovirus packaging and amplification, the purification of the adenoviral particles from cell lysate and culture medium, the viral titration, and the functional testing of the adenovirus.
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Dumitrescu, M., Trusca, V. G., Fenyo, I. M., Gafencu, A. V. An Efficient Method for Adenovirus Production. J. Vis. Exp. (172), e61691, doi:10.3791/61691 (2021).
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