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Mor-Heyecanlı Hücre-Geçirgen DNA Bağlayıcı Boya Kullanılarak Murine Spermatojenik Hücrelerin İzolasyon
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January 14, 2021
DOI:
1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute,Cincinnati Children’s Hospital Medical Center, 2Department of Pediatrics,University of Cincinnati College of Medicine, 3Division of Germ Cell Biology, National Institute for Basic Biology,National Institutes of Natural Sciences, 4Department of Basic Biology, School of Life Science,Graduate University for Advanced Studies (Sokendai), 5Department of Animal Science and Biotechnology, School of Veterinary Medicine,Azabu University, 6Faculty of Science and Technology, Department of Applied Biological Science,Tokyo University of Science
Summary
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Burada yetişkin fare testislerinden canlı meiotik ve post-meyotik mikrop hücrelerini izole etmek için basit ve etkili bir yöntem salıyoruz. Düşük sitotoksisite, menekşe heyecanlı DNA bağlayıcı boya ve floresan-aktive hücre sıralama kullanarak, bir birçok downstream uygulamaları için son derece zenginleştirilmiş spermatojenik hücre popülasyonları izole edebilirsiniz.
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Cite this Article
Yeh, Y., Hu, M., Nakagawa, T., Sakashita, A., Yoshida, S., Maezawa, S., Namekawa, S. H. Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye. J. Vis. Exp. (167), e61666, doi:10.3791/61666 (2021).
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