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Photodiode-Based Optical Imaging for Recording Network Dynamics with Single-Neuron Resolution in Non-Transgenic Invertebrates
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Biology
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JoVE Journal Biology
Photodiode-Based Optical Imaging for Recording Network Dynamics with Single-Neuron Resolution in Non-Transgenic Invertebrates

Photodiode-Based Optical Imaging for Recording Network Dynamics with Single-Neuron Resolution in Non-Transgenic Invertebrates

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2,634 Views

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10:18 min

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July 09, 2020

DOI:

10.3791/61623-v

DOI:
10.3791/61623-v

•

10:18 min
July 09, 2020

•

2 Views
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1Cell Biology and Anatomy, Chicago Medical School,Rosalind Franklin University of Medicine and Science, 2Center for Brain Function and Repair,Rosalind Franklin University of Medicine and Science

Summary

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This protocol presents a method for imaging neuronal population activity with single-cell resolution in non-transgenic invertebrate species using absorbance voltage-sensitive dyes and a photodiode array. This approach enables a rapid workflow, wherein imaging and analysis can be pursued over the course of a single day.

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Hill, E. S., Brown, J. W., Frost, W. N. Photodiode-Based Optical Imaging for Recording Network Dynamics with Single-Neuron Resolution in Non-Transgenic Invertebrates. J. Vis. Exp. (161), e61623, doi:10.3791/61623 (2020).

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