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August 06, 2020
DOI:
1Genome Biology of Neurodegenerative Diseases,German Center for Neurodegenerative Diseases (DZNE), 2Applied Genomics for Neurodegenerative Diseases,German Center for Neurodegenerative Diseases (DZNE), 3Department for Neurodegenerative Diseases, Hertie Institute for Clinical Brain Research,University of Tübingen
Summary
Automatically generated
Mostriamo l'automazione delle colture di cellule staminali pluripotenti indotte dall'uomo (hiPSC) e delle differenziazioni neuronali compatibili con l'imaging e l'analisi automatizzati.
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Automated ProductionHuman Induced Pluripotent Stem CellsCortical NeuronsDopaminergic NeuronsLive-cell MonitoringStandardized ProceduresLow Experimental VariationHigh Phenotypic ReproducibilityGraphic User InterfaceResource Instrument ProcessHEPA HoodDecontaminationUV RadiationLiquid Handling StationCulture MediumDissociation ReagentCell LineProjectBatchSeedingBrightfield Imaging CytometerCell Counting
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Dhingra, A., Täger, J., Bressan, E., Rodriguez-Nieto, S., Bedi, M., Bröer, S., Sadikoglou, E., Fernandes, N., Castillo-Lizardo, M., Rizzu, P., Heutink, P. Automated Production of Human Induced Pluripotent Stem Cell-Derived Cortical and Dopaminergic Neurons with Integrated Live-Cell Monitoring. J. Vis. Exp. (162), e61525, doi:10.3791/61525 (2020).
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