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In Vivo Intracellular Recording of Type-Identified Rat Spinal Motoneurons During Trans-Spinal Direct Current Stimulation
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Neuroscience
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JoVE Journal Neuroscience
In Vivo Intracellular Recording of Type-Identified Rat Spinal Motoneurons During Trans-Spinal Direct Current Stimulation

In Vivo Intracellular Recording of Type-Identified Rat Spinal Motoneurons During Trans-Spinal Direct Current Stimulation

English

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5,004 Views

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11:07 min

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May 11, 2020

DOI:

10.3791/61439-v

DOI:
10.3791/61439-v

•

11:07 min
May 11, 2020

•

1 Views
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1Department of Neurobiology,Poznań University of Physical Education

Summary

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This protocol describes in vivo intracellular recording of rat lumbar motoneurons with simultaneous trans-spinal direct current stimulation. The method enables us to measure membrane properties and to record rhythmic firing of motoneurons before, during and after anodal or cathodal polarization of the spinal cord.

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Here Are The Key Keywords From The Text:Intracellular RecordingSpinal MotoneuronsTrans-spinal Direct Current StimulationIn VivoRatDissectionJugular VeinTracheal TubeHind Limb NervesSciatic NerveTibial NerveGastrocnemius Nerve

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Bączyk, M., Krutki, P. In Vivo Intracellular Recording of Type-Identified Rat Spinal Motoneurons During Trans-Spinal Direct Current Stimulation. J. Vis. Exp. (159), e61439, doi:10.3791/61439 (2020).

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