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प्राथमिक न्यूरॉन्स में प्रोटीन और सिनैप्टिक मार्कर के सह-स्थानीयकरण का अध्ययन करने के लिए सुपर-रिज़ॉल्यूशन इमेजिंग
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Neuroscience
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JoVE Journal Neuroscience
Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
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प्राथमिक न्यूरॉन्स में प्रोटीन और सिनैप्टिक मार्कर के सह-स्थानीयकरण का अध्ययन करने के लिए सुपर-रिज़ॉल्यूशन इमेजिंग

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14:02 min

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October 31, 2020

DOI:

10.3791/61434-v

DOI:
10.3791/61434-v

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14:02 min
October 31, 2020

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1Department of Molecular Biochemistry and Pharmacology,Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 2Department of Neuroscience,Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 3Dulbecco Telethon Institute,Istituto di Ricerche Farmacologiche Mario Negri IRCCS

Tags

Super-resolution ImagingCo-localizationPrimary NeuronsSynaptic MarkersStructured Illumination Microscopy (SIM)Neurodegenerative DisordersHippocampal NeuronsFixationImmunostainingMounting SolutionCoverglass

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Russo, L., Natale, C., Conz, A., Kelk, J., Restelli, E., Chiesa, R., Salmona, M., Fioriti, L., Colnaghi, L. Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons. J. Vis. Exp. (164), e61434, doi:10.3791/61434 (2020).

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