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超分辨率成像研究原发神经元中蛋白质和突触标记的共同定位
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Neuroscience
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JoVE Journal Neuroscience
Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
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超分辨率成像研究原发神经元中蛋白质和突触标记的共同定位

5,609 Views

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14:02 min

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October 31, 2020

DOI:

10.3791/61434-v

DOI:
10.3791/61434-v

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14:02 min
October 31, 2020

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1Department of Molecular Biochemistry and Pharmacology,Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 2Department of Neuroscience,Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 3Dulbecco Telethon Institute,Istituto di Ricerche Farmacologiche Mario Negri IRCCS

Tags

Super-resolution ImagingCo-localizationPrimary NeuronsSynaptic MarkersStructured Illumination Microscopy (SIM)Neurodegenerative DisordersHippocampal NeuronsFixationImmunostainingMounting SolutionCoverglass

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Russo, L., Natale, C., Conz, A., Kelk, J., Restelli, E., Chiesa, R., Salmona, M., Fioriti, L., Colnaghi, L. Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons. J. Vis. Exp. (164), e61434, doi:10.3791/61434 (2020).

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