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Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System
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Neuroscience
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JoVE Journal Neuroscience
Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System

Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System

DOI:
10.3791/61237-v

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04:47 min

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May 22, 2020

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, , ,
1Department of Pharmacology,University of Colorado Denver School of Medicine

Chapters

  • 00:04Introduction
  • 00:31Cell Harvesting
  • 02:12Image Acquisition
  • 02:50Results: Point Mutation Enhances Binding
  • 04:15Conclusion

Summary

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Here, we present an optimized protocol to rapidly and semiquantitatively measure ligand-receptor interactions in trans in a heterologous cell system using fluorescence microscopy.

Tags

Protein AggregationTranscellular InteractionsCell-based AssayTrans AdhesionHeterologous Cell SystemHEK-293T CellsEDTAHemocytometerFluorescence ImagingProtein Of InterestLigand Of InterestSynaptic Ligand

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