Journal
/
Neuroscience
/
来自成人大鼠尿膀胱的原发神经元和胶质的隔离与培养
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder
Please note that all translations are automatically generated. Click here for the English version.

来自成人大鼠尿膀胱的原发神经元和胶质的隔离与培养

DOI:
10.3791/61177-v

•

06:18 min

•

May 23, 2020

•

, , , , , , ,
1School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine, 2Dongguan & Guangzhou University of Chinese Medicine Cooperative Academy of Mathematical Engineering for Chinese Medicine,Guangzhou University of Chinese Medicine, 3School of Basic Medical Sciences,Guangzhou University of Chinese Medicine

Chapters

  • 00:04Introduction
  • 00:43Bladder Harvest
  • 02:11Two-step Bladder Digestion
  • 04:14Results: Phase-contrast and Immunofluorescence Images of Primary Cells Isolated from the Rat Bladder
  • 05:36Conclusion

Summary

Automatic Translation

Automatic Translation

该协议试图建立一个可重复的协议，为初级神经元和胶质隔离从大鼠膀胱进一步细胞实验。

Tags

Primary NeuronsAdult RatUrinary BladderCell CultureCo-cultureIsolationDigestionKrebs SolutionCentrifugation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Nucleofection和胚胎小鼠海马和皮层神经元的原代培养

二层共培养小学的大鼠皮层神经元和神经胶质

从产前小鼠海马神经元的分离和培养

一个<em在体外</em从成年小鼠的肌间神经丛制备隔离肠道神经元和神经胶质

小鼠多巴胺能神经元的原代培养

分离和游离感觉神经元的文化活动从鸡胚

原发性脑多巴胺能神经元的分离，培养和长期维持胚胎脑鼠类

分离和成人神经干细胞的培养从鼠标Subcallosal区

脊髓神经元从新生小鼠中分离和培养

从E14-E16斯普拉格·道利大鼠胚胎分离的混合原发希马和皮质神经元生成神经球

Read Article