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Estabelecendo um modelo de córnea porcina ex vivo para estudar tratamentos medicamentosos contra ceratite bacteriana
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1Sheffield Collaboratorium for Antimicrobial Resistance and Biofilms (SCARAB),University of Sheffield, 2Department of Chemical and Biological Engineering,University of Sheffield, 3Hyderabad Eye Research Foundation,L V Prasad Eye Institute, 4Health Economics and Decision Science, School of Health and Related Research,University of Sheffield, 5Department of Molecular Biology and Biotechnology,University of Sheffield, 6Department of Materials Science and Engineering,University of Sheffield, 7Department of Infection, Immunity and Cardiovascular Disease,University of Sheffield
Chapters
- 00:05Introduction
- 00:35Preparation of the Corneoscleral Button
- 02:45Maintenance of the Corneoscleral Buttons
- 03:29Preparation of an Inoculum
- 04:17Infecting the Corneoscleral Button
- 05:54Homogenization of the Cornea
- 07:21Results: Ex Vivo Model Mimics In Vivo Infection
- 08:35Conclusion
Este artigo descreve um protocolo passo a passo para criar um modelo de ceratite porcina ex vivo de ceratite bacteriana. Pseudomonas aeruginosa é usado como organismo prototípico. Este modelo inovador imita a infecção in vivo, pois a proliferação bacteriana depende da capacidade da bactéria de danificar o tecido córnea.
