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Establishing a Porcine Ex Vivo Cornea Model for Studying Drug Treatments against Bacterial Keratitis
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1Sheffield Collaboratorium for Antimicrobial Resistance and Biofilms (SCARAB),University of Sheffield, 2Department of Chemical and Biological Engineering,University of Sheffield, 3Hyderabad Eye Research Foundation,L V Prasad Eye Institute, 4Health Economics and Decision Science, School of Health and Related Research,University of Sheffield, 5Department of Molecular Biology and Biotechnology,University of Sheffield, 6Department of Materials Science and Engineering,University of Sheffield, 7Department of Infection, Immunity and Cardiovascular Disease,University of Sheffield
Chapters
- 00:05Introduction
- 00:35Preparation of the Corneoscleral Button
- 02:45Maintenance of the Corneoscleral Buttons
- 03:29Preparation of an Inoculum
- 04:17Infecting the Corneoscleral Button
- 05:54Homogenization of the Cornea
- 07:21Results: Ex Vivo Model Mimics In Vivo Infection
- 08:35Conclusion
This article describes a step-by-step protocol to set up an ex vivo porcine model of bacterial keratitis. Pseudomonas aeruginosa is used as a prototypic organism. This innovative model mimics in vivo infection as bacterial proliferation is dependent on the ability of the bacterium to damage corneal tissue.
