/
/
Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
A subscription to JoVE is required to view this content. Sign in or start your free trial.
9,262 Views
•
09:57 min
•
June 14, 2020
DOI:
Summary
Automatically generated
The objective is to fractionate and isolate bioactive small molecules, peptides from a complex plant extract, and proteins from pathogenic microbes by employing liquid-phase isoelectric focusing (IEF) method. Further, the separated molecules were identified and their bioactivity confirmed.
Read Article
Cite this Article
Veerapandian, R., Paudyal, A., Chang, A., Vediyappan, G. Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method. J. Vis. Exp. (160), e61101, doi:10.3791/61101 (2020).
Copy