このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
9,260 Views
•
09:57 min
•
June 14, 2020
DOI:
概要
Automatically generated
The objective is to fractionate and isolate bioactive small molecules, peptides from a complex plant extract, and proteins from pathogenic microbes by employing liquid-phase isoelectric focusing (IEF) method. Further, the separated molecules were identified and their bioactivity confirmed.
Read Article
Cite this Article
Veerapandian, R., Paudyal, A., Chang, A., Vediyappan, G. Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method. J. Vis. Exp. (160), e61101, doi:10.3791/61101 (2020).
Copy