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Ein Femtoliter Droplet Array für massive parallele Proteinsynthese aus einzelnen DNA-Molekülen
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Chemistry
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JoVE Journal Chemistry
A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
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Ein Femtoliter Droplet Array für massive parallele Proteinsynthese aus einzelnen DNA-Molekülen

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10,073 Views

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10:45 min

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June 20, 2020

DOI:

10.3791/60945-v

DOI:
10.3791/60945-v

•

10:45 min
June 20, 2020

•

6 Views
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1SUGAR Program, X-star,Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2Center for Mathematical Science and Advanced Technology,Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 3Deep-Sea Nanoscience Research Group,Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

Summary

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Das übergeordnete Ziel des Protokolls ist es, über eine Million geordnete, einheitliche, stabile und biokompatible Femtolitertröpfchen auf einem 1 cm2 planaren Substrat herzustellen, das für die zellfreie Proteinsynthese verwendet werden kann.

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Zhang, Y., Kurosawa, K., Nishiura, D., Tei, M., Tsudome, M. A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules. J. Vis. Exp. (160), e60945, doi:10.3791/60945 (2020).

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