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April 02, 2020
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Here we describe methods using time-lapse, phase-contrast microscopy to image mouse resident peritoneal macrophages in a chemotactic complement C5a gradient. The protocols can be extended to other immune cells.
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van den Bos, E., Walbaum, S., Horsthemke, M., Bachg, A. C., Hanley, P. J. Time-lapse Imaging of Mouse Macrophage Chemotaxis. J. Vis. Exp. (158), e60750, doi:10.3791/60750 (2020).
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