A subscription to JoVE is required to view this content. Sign in or start your free trial.
22,654 Views
•
07:02 min
•
May 17, 2020
DOI:
1State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences,China Agricultural University, 2Department of Plant Biology, College of Biological Sciences,University of California, Davis, 3Genome Center, College of Biological Sciences,University of California, Davis
Summary
Automatically generated
这里描述的是一种接近标记方法，用于识别尼科蒂亚娜本查米亚叶组织中NLR免疫受体的TIR域的相互作用伙伴。还提供了一个详细的协议，用于识别其他感兴趣的蛋白质之间的相互作用使用这种技术在尼科蒂亚纳和其他植物物种。
Read Article
Cite this Article
Zhang, Y., Li, Y., Yang, X., Wen, Z., Nagalakshmi, U., Dinesh-Kumar, S. P. TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks. J. Vis. Exp. (159), e60728, doi:10.3791/60728 (2020).
Copy