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Isolamento, cultura e indução adipogênica de células-tronco originais de adipose da crista neural do tecido adipogênico periaorótico
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March 02, 2020
DOI:
1Department of Cardiology, Shanghai Chest Hospital,Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences,Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension,Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine,Huadong Hospital Affiliated to Fudan University
Summary
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Apresentamos um protocolo para o isolamento, cultura e indução adipogênica de células-tronco derivadas de adipose derivados da crista neural (NCADSCs) do tecido periártico adiposo de Wnt-1 Cre+/-; Rostou rosa26RFP/+. Os NCADSCs podem ser uma fonte de ADSCs facilmente acessível para modelar adipogênese ou lipogênese in vitro.
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Qi, Y., Miao, X., Xu, L., Fu, M., Peng, S., Shi, K., Li, J., Ye, M., Li, R. Isolation, Culture, and Adipogenic Induction of Neural Crest Original Adipose-Derived Stem Cells from Periaortic Adipose Tissue. J. Vis. Exp. (157), e60691, doi:10.3791/60691 (2020).
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