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Journal
/
Cancer Research
/
正交胰腺肿瘤的产生与肿瘤渗透T细胞细胞毒性的
外体
特征
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
Generation of Orthotopic Pancreatic Tumors and
Ex vivo
Characterization of Tumor-Infiltrating T Cell Cytotoxicity
Please note that all translations are automatically generated.
Click here for the English version.
正交胰腺肿瘤的产生与肿瘤渗透T细胞细胞毒性的
外体
特征
DOI:
10.3791/60622-v
•
06:16 min
•
December 07, 2019
•
Sarah Spear
2
,
Iain A McNeish
,
Melania Capasso
3
1
Division of Cancer, Department of Surgery and Cancer
,
Imperial College London
,
2
Centre for Cancer and Inflammation, Barts Cancer Institute
,
Queen Mary University of London
,
3
German Center for Neurodegenerative Diseases (DZNE)
Chapters
00:04
Title
00:37
Digestion of Pancreatic Tumors
01:24
Preparation of Single-cell Suspension from Digested Tumor
03:04
Preparing Cells for
Ex Vivo
Stimulation
03:57
Results: Flow Cytometry Analysis and Quantification of
Ex Vivo
Spleen- and Tumor-derived T Cell Activity
05:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了正交胰腺肿瘤的外科生成和新鲜分离的鼠胰腺肿瘤的快速消化。消化后,可行的免疫细胞群可用于进一步下游分析,包括通过流式细胞测定检测细胞内细胞因子的T细胞
外体
刺激。
Tags
Orthotopic Pancreatic Tumors
Tumor-infiltrating T Cells
Cytotoxicity
Tumor Digestion
Ex Vivo Characterization
Therapeutic Agents
T Cell Response
Flow Cytometry
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