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Chapters
- 00:04Title
- 00:58Organotypic Cerebellar Slice Culture Preparation
- 01:40Organotypic Cerebellar Slice Culture Dissection
- 03:32Immunofluorescence
- 05:24Results: Representative Purkinje Cell Survival in Organotypic Cerebellar Slice Culture Following High Potassium Chloride (KCl) Treatment
- 05:54Conclusion
Organotypic skive kulturer er et kraftig verktøy for å studere neurodevelopmental eller degenerative/regenererende prosesser. Her beskriver vi en protokoll som modeller den neurodevelopmental død Purkinje celler i mus lillehjernen skive kulturer. Denne metoden kan ha nytte forskning i nervecellene narkotika funn.
