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细菌细胞的三维成像，实现精确的细胞表现和精确的蛋白质定位
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
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细菌细胞的三维成像，实现精确的细胞表现和精确的蛋白质定位

English

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9,385 Views

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06:33 min

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October 29, 2019

DOI:

10.3791/60350-v

DOI:
10.3791/60350-v

•

06:33 min
October 29, 2019

•

4 Views
, ,
1Department of Microbiology and Molecular Genetics,Oklahoma State University, 2Department of Molecular Biology and Lewis-Sigler Institute of Integrative Genomics,Princeton University

Summary

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该协议解释了如何准备和安装细菌样本进行活的三维成像，以及如何从这些图像中重建大肠杆菌的三维形状。

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Bratton, B. P., Barton, B., Morgenstein, R. M. Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization. J. Vis. Exp. (152), e60350, doi:10.3791/60350 (2019).

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