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Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK

Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK

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9,143 Views

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11:20 min

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December 19, 2019

DOI:

10.3791/60294-v

DOI:
10.3791/60294-v

•

11:20 min
December 19, 2019

•

13 Views
, , , , ,
1Institute of Neurobiology,Heinrich Heine University Düsseldorf, 2Department of Neurology,Düsseldorf University Hospital

Summary

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We describe a protocol for cell-type specific expression of the genetically encoded FRET-based sensor ATeam1.03YEMK in organotypic slice cultures of the mouse forebrain. Furthermore, we show how to use this sensor for dynamic imaging of cellular ATP levels in neurons and astrocytes.

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Lerchundi, R., Kafitz, K. W., Färfers, M., Beyer, F., Huang, N., Rose, C. R. Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK. J. Vis. Exp. (154), e60294, doi:10.3791/60294 (2019).

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