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Mikroinjektion von DNA in Augenknospen in Xenopus laevis Embryonen und Bildgebung von GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles
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Developmental Biology
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JoVE Journal Developmental Biology
Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles
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Mikroinjektion von DNA in Augenknospen in Xenopus laevis Embryonen und Bildgebung von GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

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6,078 Views

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06:32 min

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September 04, 2019

DOI:

10.3791/60123-v

DOI:
10.3791/60123-v

•

06:32 min
September 04, 2019

•

2 Views
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1College of Osteopathic Medicine,Touro University California

Summary

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Dieses Protokoll soll zeigen, wie man ein DNA/DOTAP-Gemisch in Augenknospen von eintägigen Xenopus laevis Embryonen mikroinjiziert und wie man einzelne grüne fluoreszierende Proteine (GFP) abbilden und rekonstruieren kann, die optische axonale Lauben in tektalen Midhirnen von intakte, lebende Xenopus Kaulquappen.

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MicroinjectionDNAXenopus LaevisEmbryosGFPOptic Axonal ArborsTadpolesTransgenesisCell-specificVertebrate ModelGlass MicropipetteMineral OilDOTAPDe-vitellinizationEmbryo Transfer

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Cite this Article

Dao, S., Jones, K., Elul, T. Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles. J. Vis. Exp. (151), e60123, doi:10.3791/60123 (2019).

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