Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Yafeng Zhang; Xun Liao; Peihua Luo; Kui Peng; Wenyi Ye; Tengxiang Lian; Qibin Ma; Hai Nian

doi:10.3791/60019

/

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

JoVE Journal
Genetics

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Genetics

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

6,034 Views

•

07:26 min

•

July 29, 2019

DOI:

10.3791/60019-v

DOI:

10.3791/60019-v

•

07:26 min

July 29, 2019

•

2 Views

Yafeng Zhang^1,2, Xun Liao², Peihua Luo², Kui Peng², Wenyi Ye², Tengxiang Lian^1,2, Qibin Ma^1,2, Hai Nian^1,2

¹State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources,South China Agricultural University, ²Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture,South China Agricultural University

Summary

We present a circular RT-PCR-based strategy by combining circular RT-PCR, quantitative RT-PCR, RNA 5' polyphosphatase-treatment, and Northern blot. This protocol includes a normalization step to minimize the influence of unstable 5' triphosphate, and it is suitable for discriminating and mapping the primary and processed transcripts stably accumulated in maize mitochondrion.

Explore More Videos

Mitochondrion Maize Primary Transcripts Processed Transcripts Circular RT-PCR RNA Normalization Five Prime Polyphosphatase Treatment RNA Extraction Reverse Transcription PCR

Read Article

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

Automatically Generated

Summary

Automatically generated

Explore More Videos