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分层和可编程单罐寡糖合成
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Chemistry
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JoVE Journal Chemistry
Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
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分层和可编程单罐寡糖合成

DOI:
10.3791/59987-v

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09:56 min

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September 06, 2019

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, , , , , ,
1Genomics Research Center,Academia Sinica, 2Institute of Biomedical Sciences,Academia Sinica, 3Department of Chemistry,Scripps Research Institute, 4Institute of Information Science,Academia Sinica

Chapters

  • 00:04Title
  • 01:15Auto-CHO Software Manipulation
  • 05:08RRV Determination Experiments
  • 07:29Results: The [2 + 1 + 3] One-pot Synthetic Strategy for SSEA-4
  • 08:46Conclusion

Summary

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该协议演示如何使用 Auto-CHO 软件进行寡糖的分层和可编程单罐合成。还介绍了RV测定实验和SSEA-4单罐糖基化的一般程序。

Tags

Hierarchical And Programmable One-Pot Oligosaccharide SynthesisAuto-CHO SoftwareOligosaccharide SynthesisCarbohydrate-based TherapeuticsGlycan StructureBuilding Block SelectionReactivity Value PredictionOne-pot SynthesisFragment Composition

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